Nucleic acid diagnostics
Project leader: Ulla Karhunen, MSc
Researchers
Project description: The research activities of the nucleic acid diagnostics group are focused on lanthanide chelate based assay concepts using a time-resolved mode of measurement in closed compartments, novel nucleic acid detection systems using dual-tag lanthanide chelate sensors, simple sample pre-treatment methods, and ready-to-use dry reagents using inexpensive chip formats. The present targets of the assays are microbes that cause infections.
We coordinate the three-year research project ACUSEP (December 2010 – November 2013) funded by EU’s Seventh Framework Programme.
Publications
Karhunen, U., Rosenberg, J., Lamminmäki, U., Soukka, T. Homogeneous Detection of Avidin Based on Switchable Lanthanide Luminescence. (2011) Anal Chem 83:9011-9016.
Lehmusvuori, A., Juntunen, E., Tapio, A.-H., Rantakokko-Jalava, K., Soukka, T., Lövgren, T. Rapid homogeneous PCR assay for the detection of Chlamydia trachomatis in urine samples. (2010) J Microbiol Meth 83:302-306.
Karhunen, U., Jaakkola, L., Wang, Q., Lamminmäki, U., Soukka, T. Luminescence switching by hybridization-directed mixed lanthanide complex formation. (2010) Anal Chem 15: 751-754.
Hagrèn V., Lode P., Syrjälä A., Soukka T., Lövgren T., Kojola H., Nurmi J. An automated PCR platform with homogenous time-resolved fluorescence detection and dry chemistry assay kits. (2008) Anal Biochem 374: 411–416.